【陈诉标题】Non-invasive optical characterization of cells and tissues using endogenous contrast
Disruption of cell-matrix interactions is associated with a wide range of diseases such as cancer, fibrosis, and neurodegenerative diseases. Modified interactions have major effects upon key functional cellular features, as well as upon the matrix biophysical and biochemical properties. In this talk, I will focus on my recent work of improved characterization of these structure-function connections based on endogenous contrast of cells and tissues. With regards to the cellular features, we use two-photon excited fluorescence (TPEF) microscopy to acquire images from conenzymes of NADH and FAD which are actively involved in a number of metabolic pathways, and develop novel optical biomarkers to characterize cell features, including the optical redox ratio, phasor fluorescence lifetime and mitochondrial clustering. On the other hand, we use second harmonic generation (SHG) approach to image collagen fibers in a 3D context. We develop 3D orientation and organization algorithms to characterize the spatial alignment of collagen, which proves to be a sensitive biomarker in several diseases, such as cancer and traumatic brain injury. Overall, these improvements in cell and matrix characterization lead to a better understanding of cell-matrix interactions.
【陈诉人简介】刘智毅，博士，2013年获清华大学理学博士学位，之落伍入美国塔夫茨大先生物医学工程系展开博士后研讨至今，导师为Irene Georgakoudi传授。次要研讨偏向为生物医学光子学，包罗双光子荧光成像，荧光寿命成像，二次谐波成像等技能及在疾病诊断中的使用，并开展了相应的图像处置办法和表征参量。已发布SCI论文20余篇，此中包罗Biomaterials, Biosensors & Bioelectronics品级一著作人或通讯著作人的论文10篇；在协作著作人论文中，包罗Science Translational Medicine 的封面文章等。担当Chemical Communications, Journal of Biophotonics, Biomedical Optics Express等杂志的审稿人。